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At least 38 people were killed in Myanmar on Wednesday in what the UN described as the "bloodiest day" since the coup took place a month ago.
UN envoy to Myanmar Christine Schraner Burgener said there was shocking footage coming out of the country.
Witnesses said security forces opened fire with rubber and live bullets.
Mass protests and acts of civil disobedience have been seen across Myanmar since the military seized power on 1 February.
Protesters have been calling for an end to military rule and the release of the country's elected government leaders - including Aung San Suu Kyi - who were overthrown and detained in the coup.
The coup and the violent suppression of protests that followed have led to international condemnation, which Myanmar's military has so far ignored.
Reacting to Wednesday's deaths, the UK called for a United Nations Security Council meeting on Friday, while the US said it was considering further action against Myanmar's military.
The latest violence comes a day after Myanmar's neighbours urged the military to exercise restraint.
'They just came out and started to shoot'
Ms Schraner Burgener said at least 50 people had now been killed "and many wounded" since the coup began.
She said one video clip showed police beating an unarmed volunteer medical crew. Another showed a protester being shot and probably killed on the street, she said.
"I asked some weapons experts and they could verify to me, it's not clear but it seems that the police weapons like 9mm submachine guns, so live ammunition," she said.
Reports from inside Myanmar spoke of security forces opening fire on large crowds in a number of cities, including Yangon, with little warning.
Two boys, aged 14 and 17, were among those who were killed, Save the Children said. A 19-year-old woman was also said to be among the dead.
Image source, Getty Images
Image source, Reuters
At least six people were reportedly shot dead during a protest in Monywa in central Myanmar. At least 30 others were wounded in the unrest, a local journalist told Reuters.
A volunteer medic told AFP news agency in Myingyan that at least 10 people had been injured there. "They fired tear gas, rubber bullets and live rounds," they said.
"They didn't spray us with water cannon, [there was] no warning to disperse, they just fired their guns," one protester in the city told Reuters.
In Mandalay, a student protester told the BBC that demonstrators were killed near her house.
"I think around 10am or 10.30, police and soldiers came to that area and then they started to shoot at civilians. They didn't give any warning to the civilians.
"They just came out and they started to shoot. They used rubber bullets but they also used live bullets to kill civilians in a violent way."
The military has not commented on the reported deaths.
Military stands firm despite pressure
As world powers view Myanmar's crisis with growing unease, the country's military said it was ready to withstand sanctions and isolation after its coup,
Ms Schraner Burgener has urged the UN to take "very strong measures" against the generals. The UN envoy warned of such punitive action in a conversation with Myanmar's deputy military chief.
In response, "the answer was: 'We have to learn to walk with only few friends'," Ms Schraner Burgener told reporters in New York.
Nevertheless, more sanctions are being considered by Western countries, including the US.
Image source, Reuters
Wednesday's violence left the US "appalled", State Department spokesman Ned Price said. He told reporters: "We call on all countries to speak with one voice to condemn the brutal violence by the Burmese military against its own people."
He urged China, historically a close ally of Myanmar, to bring its influence to bear on the country's military.
The UN Security Council - an international peacekeeping body - has voiced concern over the situation, but stopped short of condemning the coup because of opposition by Russia and China, who view the matter as an internal affair.
Meanwhile, Pope Francis called for dialogue to "prevail over repression".
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End of twitter post by Pope Francis
Foreign ministers of neighbouring South East Asian nations held a special meeting on Tuesday about the situation in Myanmar.
But although they urged restraint, only some of the ministers pressed the military junta to release Ms Suu Kyi.
Ms Suu Kyi, 75, was seen for the first time since her detention earlier this week when she appeared in court via video link.
The military says it seized power because of alleged fraud in November's general elections, which saw Ms Suu Kyi's National League for Democracy (NLD) party win a landslide victory.
But the military has provided no proof of these allegations - instead, it replaced the Election Commission and promised fresh polls in a year.
Myanmar in profile
Myanmar, also known as Burma, became independent from Britain in 1948. For much of its modern history it has been under military rule
Restrictions began loosening from 2010 onwards, leading to free elections in 2015 and the installation of a government led by veteran opposition leader Aung San Suu Kyi the following year
In 2017, Myanmar's army responded to attacks on police by Rohingya militants with a deadly crackdown, driving more than half a million Rohingya Muslims across the border into Bangladesh in what the UN later called a "textbook example of ethnic cleansing"
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